Mouse brain
Automated cell counter analytical reporter: Cell viability is 93%. Cell yield is 1.03 x 10^6/100mg.
Flow cytometry analysis of mouse brain
The kit digests tissue to isolate neurons, endothelial cells, microglia, oligodendrocytes, astrocytes.
Primary culture
The primary cells are in good condition and can be seen growing adherently.
Q1:What are the storage conditions for the tissue enzymatic digestion kit?
A1:Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions. The debris removal reagent in the adult brain kit must be stored at 4°C in the refrigerator, as freezing may cause the reagent to lose its effectiveness.
Q2:What are the pre-treatment precautions for tissue samples?
A2:During the sampling process, carefully remove the blood vessels and residual blood clots, and rinse the tissue thoroughly.
Q3:What is the suitable mouse age for the adult brain kit?
A3:Mice of any size older than seven days of age (P>7).
Astrocytic LRP1 enables mitochondria transfer toneurons and mitigates brain ischemic stroke bysuppressing ARF1 lactylation. Zhou J, Zhang L, Peng J, Zhang X, Zhang F, Wu Y, Huang A, Du F, Liao Y, He Y, Xie Y, Gu L, Kuang C, Ou W, Xie M, Tu T, Pang J, Zhang D, Guo K, Feng Y, Yin S, Cao Y, Li T, Jiang Y. Cell Metab. 2024 Sep 3;36(9):2054-2068.e14. doi: 10.1016/j.cmet.2024.05.016.
IF:27.7/Q1
Protocol for transplantation of cells derivedfrom human midbrain organoids into aParkinson’s disease mouse model to restoremotor function. Fu CL, Jiang X, Dong BC, Li D, She XY, Yao J. STAR Protoc. 2024 Aug 8;5(3):103251. doi: 10.1016/j.xpro.2024.103251.
IF:1.3/Q4