Flow cytometry analysis
Cell viability is 99.76%. MSCs (Mesenchymal Stem Cells) population is selected by surface markers CD73+, CD90+, CD45-, and CD34-.
Cell Culture
Cells start to adhere at P0-8d. After subculture, the cell growth ability is significantly enhanced, and the cells present a uniform long spindle shape.
Q1: What are the storage conditions for the tissue enzymatic digestion kit?
A1: Different kits contain different types of enzymes. Store them separately in a refrigerator at 4°C or -20°C according to the storage requirements in the instructions.
Q2: What are the precautions for the pretreatment of tissue samples?
A2: During the sampling process, carefully remove blood vessels and residual blood clots, and repeatedly rinse the tissue until it is clean.
Q3: What are the main types of target cells for extraction?
A3: Umbilical cord mesenchymal stem cells can be obtained.