Overview
Fiber photometry calcium imaging is becoming increasingly popular in neuroscience research and, based on its advantages, is now an indispensable tool for the real-time detection of neural signals.
R810 Dual Color Multichannel Fiber Photometry System has two excitation light sources (410nm and 470nm). The 410 nm can be used to reflect the background noise signal, thus ensuring the acquisition of true fluorescence data.
R820 Tri-Color Multichannel Fiber Photometry System has three excitation light sources (410nm, 470nm and 560nm).The system can record signal of green fluorescence indicator like GCaMP and dLight or neurotransmitter probe and red fluorescence indicator like RCaMP, jrGECO1a or neurotransmitter probe.
Main Applications:
- Ca2 + and neurotransmitter signal detection
- Study the function of neural circuits
- Explore the mechanisms of neurological diseases
- Develop new fluorescent sensor probe
- Experiment of optical principle
Principle:
Fiber photometry is a technology to detecting the activity of neurons in the brain nucleus of freely moving animals. It sums up the overall fluorescence of neurons expressing a genetically encoded calcium indicator(GECI) or neurotransmitter probes.
Fiber photometry calcium imaging has been in development for nearly a decade and has been highly rated by many laboratories. It can be used to explore the regulatory mechanisms behind animal behavior.
In fiber photometry, data are collected by analyzing the fluorescence change (ΔF) relative to the initial baseline fluorescence (F) and observing the signal change corresponding to the calcium transient (ΔF/F). These metrics are usually based on fluorophores such as GFP, RFP, tdTomato, mCherry, etc., of which GCaMP is the most common example.
In neurons, calcium ions regulate several important processes, including neurotransmitter release and membrane excitability. Fiber photometry calcium imaging can provide critical insight into calcium dynamics, as GCaMP fluoresces only when bound to calcium ions. Based on similar principles, fiber photometry systems can also detect changes in the concentration of neurotransmitters.
(Neurotransmitter probes – cpEGFP is embedded in a specific neurotransmitter receptor, and the binding of the receptor to the neurotransmitter triggers a conformational change in the receptor to convert to a fluorescent signal, and this genetically encodable probe is expressed in cells or mouse brain by viral injection, transfection, and other technical means.)
