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RWD Magnetic Microbeads Cell Separation Kits include self-developed magnetic microbeads cell separation kits and cell separation columns, which can obtain target cells with high purity and high viability through simple operation. Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, etc.
Application:
T cell separation, B cell separation, immune cell separation, microglia separation, (stem cell separation)
1.The process is simple and clear， and the process only takes 25 minutes.
2.Self-developed 50nm small magnetic beads, biodegradable organic materials were selected to achieve gentle and non-destructive sorting, and the target cells with high viability and multiple epitopes were obtained.
| Cell Type | Cat. No | Product Name | Size/Capacity | |
|---|---|---|---|---|
| T cells | K1301-10 | Mouse CD3+ Cell Separation Kit | 1ml*2/for 1*10^9 total cells | Learn more |
| T cells | K1302-10 | Mouse CD4+ Cell Separation Kit | 1ml*2/for 1*10^9 total cells | Learn more |
| T cells | K1303-10 | Mouse CD8+ Cell Separation Kit | 1ml*2/for 1*10^9 total cells | Learn more |
| Leukocytes | K1304-10 | Mouse CD45+ Cell Separation Kit | 1ml*2/for 2*10^9 total cells | Learn more |
| B cells | K1305-20 | Mouse CD19+ Cell Separation Kit | 1ml*2/for 2*10^9 total cells | Learn more |
| Myeliod cells | K1306-10 | CD11b+ (Microglia) cell separation Kit | 1ml*2/for 1*10^9 total cells | Learn more |
| T cells | K1201-10 | Human CD3+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| T cells | K1202-10 | Human CD4+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| T cells | K1203-10 | Human CD8+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| Monocytes | K1204-10 | Human CD14+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| Leukocytes | K1205-10 | Human CD14+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| B cells | K1206-10 | Human CD19+ Cell Separation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| NK cells | K1207-10 | Human NK Cell Isolation Kit(RUO) | 1ml*2/for 1*10^9 total cells*2 | Learn more |
| / | K1200-10 | Streptavidin Nano-Magnetic beads（RUO） | 1ml*1/for 1*10^9 total cells | Learn more |
Currently available cell separation kits can perform 1*10^9 or 2*10^9 cell separation per box. Take the treatment of mouse spleen to obtain immune cells as an example: each mouse spleen has about 1*10^8 cells, so a kit can be used to sort spleen immune cells of about 10 mice.
The amount of kit is calculated according to the proportion of cells of different purpose in the tissue, for example, there are about 1*10^8 cells in the spleen or bone marrow cells of a mouse. Among them, CD3+ T cells accounted for about 30%, CD4+ T cells accounted for about 20%, CD8+ T cells accounted for about 10%, B cells accounted for about 60%, and NK cells accounted for about 1%. In addition, in human peripheral blood PBMC, there are about 1-2*10^6 cells per milliliter of whole blood, CD3+T cells account for about 45-70%, CD4+T cells account for about 10-40%, CD8+T cells account for about 2-20%, and CD14+ monocytes account for about 10-30%. NK cells accounted for about 5-10%, and B cells accounted for about 5-15%.
CDn is a type of antigen on the cell surface, and + represents the positive detection of this antigen, such as CD3+ represents mature T lymphocytes, CD4+ represents induced T cells (Ti)/ helper T cells (Th), the most important hub cell for regulating immune response; CD8+ stands for inhibitory T cells (Ts)/ Cytotoxic T cells (Tc), the direct killer cells in the immune response. The ratio of CD4+ to CD8+ is a sensitive index for clinical diagnosis of human immune dysfunction. If the ratio is greater than 2.5, it indicates that the cellular immune function is in an “overactive” state and is prone to autoimmune reaction. The decrease of this value is an important sign of the decline of immune function.
The magnetic beads used in our kit are streptavidin magnetic beads, which can be used with any biotinylated antibody. You can adjust the ratio and dosage of antibody and magnetic beads according to your needs for related experiments. The magnetic bead will be officially commercialized later, if the customer has a universal magnetic bead demand for private technical personnel/product managers.
The water and particle size of the nano-magnetic beads are only 50nm and will fall off with the cell culture process, about 2-3 days, cell culture and activation experiment data can prove that the magnetic beads have no effect on the state of the cells.
