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Optimized enzymatic hydrolysis formulation for efficient and gentle digestion of mouse tumor tissues, preserve the integrity of surface antigenic epitopes.
Cat No: DHTE-5001
Number of reactions: 50T
Mouse colon cancer
Automated cell counter analytical reporter: Cell viability is 95%. Cell yield is 10 x 10^6/100mg.
Mouse breast cancer
Automated cell counter analytical reporter: Cell viability is 98%. Cell yield is 30 x 10^6/100mg.
Flow cytometry analysis of immune cells in mouse tumor tissues
Single cell suspensions from tumor tissues can be sorted into immune cell subsets by the surface antigens CD45, CD3, CD4, CD8, CD44.
Q1:What are the storage conditions for the tissue enzymatic digestion kit?
A1:Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions.
Q2:What are the pre-treatment precautions for tissue samples?
A2:During the sampling process, carefully remove the blood vessels and residual blood clots, and rinse the tissue thoroughly.
Q3:What are the specific requirements for preparing single-cell suspension from mouse tumor tissue?
A3:First, confirm the tissue type. If it is a human-derived mouse xenograft, you need to apply for a human tumor kit. For the processing methods of specific tissues, you need to refer to the instructions of the corresponding enzymatic digestion kit.
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