High Efficiency Nuclei Extraction Kit

RWD high-efficiency nuclear extraction kit is specially designed for extracting pure nuclei from mammalian cells and tissues. It can efficiently and quickly obtain a uniform, intact nucleus suspension with few debris from fresh and frozen tissue samples.

Cat No: DHNE-2511

Number of reactions: 25T

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Technical Specifications

Data Support
Q&A
Related Resources

Number of Cell Nucleus

For nuclear extraction from fresh or frozen mouse spleen, mouse kidney, mouse liver, and A549 cell line-induced tumors, the yields of extracted nuclei are all greater than 10^4 nucleus/mg.

Nucleus suspension with uniformity, integrity and few debris

Q1: What are the storage conditions for tissue enzymatic digestion kits?

A1: Different kits contain different types of enzymes. They should be stored in a refrigerator at 4°C or – 20°C according to the storage requirements stated in the user manual.

Q2: What are the types of tissues suitable for nuclear extraction?

A2: Fresh or frozen tissue samples of mammals, such as tissues of the heart, liver, spleen, lung and kidney.

Q3: What is the principle of nuclear extraction by this kit?

A3: Used in combination with the RWD Single Cell Suspension Dissociator, nuclei are extracted through a method that combines mechanical cutting and tissue lysis. The RWD Single Cell Suspension Dissociator mainly plays a role in mechanical dissociation, while the High Activity Adipose Tissue Enzymatic Digestion Kit mainly lyses cells to release nuclei.

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