Perform the two-step perfusion method on mice and rats of different strains respectively. Extract hepatocytes by in vivo perfusion. The extracted cells have high viability, high yield and stable results.
Performing the two-step perfusion method on mice to extract hepatocytes by in vivo perfusion can meet the downstream primary hepatocyte culture experiment, and the adherent growth state is good.
Q1: What are the storage conditions for the tissue enzymatic digestion kit?
A1: Different kits contain different types of enzymes. They should be stored separately in a refrigerator at 4℃ or – 20℃ according to the storage requirements stated in the instructions.
Q2: What is the experimental method for extracting hepatocytes using the mouse and rat hepatocyte extraction kit?
A2: Hepatocytes are extracted by in vivo perfusion through a two step perfusion method.
Q3: What are the main types of target cells for extraction?
A3: Both hepatocytes and other non parenchymal cells can be extracted.