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| Guide cannula | Internal cannula | Fixing screw | Dummy cannula | Cap | PU tube | Plug | Screw |
| Guide cannula consists of a cylindrical base with threads and a thin stainless steel tube. It is pre-implanted intracranially and secured with dental cement around the injection orifice. Both dummy cannula and internal cannula can be inserted into the guide cannula individually for sealing or injections. | Internal cannula connects with the guide cannula on one end and with PU tube on the other end for injections. | Fixing screw is used to fix the relative position of the internal cannula and the guide cannula to ensure stable connection and avoid leakage. | Dummy cannula is composed of a stainless steel wire and a threaded plastic cap. For use, it should be inserted in and fixed on the guide cannula to prevent tissue fluid from entering the catheter and causing it to become blocked. The length of the stainless steel wire can be customized or cut according to actual needs.Optional plastic or metal material (more durable). | The Dummy Cannula of double cannula is divided into two parts: Dummy Cannula and Cap，easy to tighten and secure. | PU tube connects with the internal cannula on one end and syringe on the other end for drug injection. | Plug connects with PU tube on the one end and with syringes of different sizes on the other end. | Screw provides the dental cement with a point of strength to prevent it from falling off. |
A – The length of the stainless steel tubing below the plastic pedestal
B – Height of cylindrical plastic pedestal，which is 7.8mm for Series I, II & IV and 5.5mm for Series III
C – The length of the stainless steel tubing below the plastic pedestal,can be customized.
C.C-Center distance between double guide cannulas, double dummy cannulas and double injectors. These 3 values should be the same in one system.
D – External diameter of stainless steel guide cannulas.
E – External diameter of cylindrical plastic base.
F1 – External diameter of Fixing screw.
F2 – External diameter of Dummy Cannulas.
H1 – Height of Fixing screw.
H2 – Height of Dummy Cannulas.
G1- The internal cannulas projection length beyond the guide cannulas tip, can be customized.
G2- The dummy cannulas projection length beyond the guide cannulas tip, can be customized.
Series I – Rats/Mouse
Series II – Rats
Series III – Mouse
Series IV – Mouse
Purchasing Steps：
Step 1：
The type of guide cannula-double is first selected based on the experimental animal and the depth of two target brain regions.
For Series I, the guide cannula-double has the specification and outer diameter of 26 G and 0.48mm respectively, suitable for both rat and mice.
For Series II, the guide cannula-double has the specification and outer diameter of 23 G and 0.64mm respectively, suitable for rat.
For Series III, the guide cannula-double has the specification and outer diameter of 26 G and0.48 mm respectively, suitable for mice.
For Series IV, the guide cannula-double has the specification and outer diameter of 27 G and0.41 mm respectively, suitable for mice.
NOTE: The Series III and Series I guide cannula-double have different height of cylindrical plastic pedestal (7.8mm for the former and 5.5mm for the latter) and Series III guide cannula-double is only suitable for mice.
Step 2：
Determine the C.C value: the C.C value should be chosen according to the horizontal distance between the two target brain regions.
Step 3：
Determine the C value: length of the stainless steel tube of the Guide cannula. C = depth of target brain region + thickness of dental cement (0.5 ~ 0.8 mm) – G1.
Step 4：
Determine the G1 value: the length of the protruding part of the internal cannula when it is inserted into the guide cannula.Usually 0.5 mm or 1.0 mm is selected, and 0.5 mm is recommended.
Step 5：
Determine the G2 value: the length of the protruding part of the dummy cannula when it is inserted into the guide cannula.Usually 0, 0.5mm or 1.0 mm is selected, 0 mm is recommended.
Series I: Recommended for rat or mouse
Series II: Recommended for rat
Series III: Recommended for Mouse
Series IV: Recommended for Mouse
Screws：
| Model | Product Description |
| 62513 | Screw-M1.0xL2.0mm |
Note:
(1) For cap, plastic or metal is optional. Metal cap 62525 (corresponding plastic cap 62523).
(2) The difference between SeriesIand Series III is the height of the pedestal, the former is 7.8mm and latter is 5.5mm, and Series III is specially designed for mice.
(3) For double cannula, the 68205 holder is suitable for a pedestal with a diameter of 3.5mm, whereas the 68230 holder is suitable for one with a diameter of 5.5mm.
Laboratory Animals Brain Single Cannula
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