Using fiber photometry to detect dynamic changes in calcium signaling in LDT (GABA) neurons of awake mice under different sensory stimuli

Du, Yonglan et al. “Dopamine release and negative valence gated by inhibitory neurons in the laterodorsal tegmental nucleus.” Neuron vol. 111,19 (2023): 3102-3118.e7. doi:10.1016/j.neuron.2023.06.021

Demand of the Product：we utilized in vivo fiber photometry to measure calcium (Ca2+) dynamics of LDTGABA neurons in awake behaving mice, during the presentation of diverse sensory stimuli. And we combine optogenetic modulation of excitation/inhibition in LDTGABA neurons with fiber photometry of DA release in NAcLat

Journal: Neuron

Animal: Mice

Virus Injection Site: LDT（Laterodorsal Tegmental Nucleus)

Research Highlight: This study finds two separate populations of LDT(GABA) neurons are involved in the regulation of distinct behavioral reactions to unpleasant stimuli

Research Method: Stereotaxic surgery、Tracing、Fiber photometry、Optogenetic stimulation、Two-photon、Miniscope、Patch-clamp recording、Immunohistochemistry、CPP task、Open field、Real-time place preference test、Von Frey test、Hot plate test